Uji Sitotoksik Senyawa

7/25/2019 Uji Sitotoksik Senyawa

1/30


2/30

LATAR BELAKANG

Gamba 1! P"#$"bab %"ma&'a# (' ()#'a


3/30

LATAR BELAKANG

Gamba 2! I#*'("#*' (a# m+&a,'&a* %a#%" (' ()#'a


4/30

LATAR BELAKANG

Gamba ! I#*'("#*' (a# m+&a,'&a* %a#%" (' ()#'a


5/30

P"#.+ba&a#%a#%" P"mb"(aa# Ra('a*'

K"m+&"a' mustard gasalkylating agents3

#)&'*'aminopterin3

a#&'b'+&'%streptomycin3

E"% *am'#. K",",aa# M)a, (a# m)#&a N$"' Ramb)& +#&+%

A#"m'a I#"%*' Ma*a,a "mb"%)a# (aa Ma*a,a +#..a m),)& .)*' (a#

&"#..++%a# D'a" (a# %+#*&'a*' E"% a(a *aa (a# +&+& E"% a(a %),'& (a# %)%) Radiation recall E"% .'#a, (a# %a#()#. %"m' G"a,a m"#$")a' )

R"&"#*' 8a'a# E"% a(a +.a# *"%*)a, (a# *"%*)a,'&a*

LATAR BELAKANG


6/30

ISO9LA:ON K",+m+% *"#$a;a

a


7/30

P"#",'&'a# *"b",)m#$a E)."#+, '*+,a& %"(",a'

m",a;a# %a#%"a$)(aa

"#$a%'& a#&)#. (a#

"mb),) (aa

."a,a +*&m"#+a)*a,

E)."#+, '*+,a& 8"#.%" M"#.amba& +,'"a*'

*",

I#()%*' a+&+*'*

P"#",'&'a# '#' E)."#+, '*+,a&

m'#$a% (a)#

8"#.%"a%&'


8/30

RUMUSAN MASALAH

Ba.a'ma#a a%&'


9/30

TU@UAN PENELITIAN

M"#."&a)' a%&'


10/30

MAN9AAT PENELITIAN

P"#",'&'a# '#' ('%"mba#.%a# )#&)%m"#8a' *"#$a;a a#&'%a#%" ba) $a#.,"b' *"#*'&' (a# ,"b' *"*'%! S"&",a('%"&a)' a%&'


11/30

TIN@AUAN PUSTAKA

NEOPLASIA D"#'*' ma**a ab#+ma, *)a&) a'#.a# $a#.

&)mb) m","b'' (a# &'(a% &"%++('#a*' (a'a'#.a# #+ma, *"&a &"&a a(a (a,am b"#&)%

m",ama)' ba&a* *"&",a "#."#&'a# *&'m),)*$a#. m"$"bab%a# &'mb),#$a ")baa# &"*"b)&!Da,am oncology,#"+,a*'a ('ba.' m"#a(' '#a% (a#.a#a*! T)m+ .a#a* *"8aa %+,"%&' ('*"b)& %a#%"!

Ka#%" m")a%a# .a#..)a# "&)mb)a# (a#"',a%) *", *"'#..a '("#&'%a*' ba'% (a' &'#.%a&*",),a ma))# m+,"%)," "#$"bab +%+% m"#a('a, $a#. "#&'#.!


12/30

Ka#%" a$)(aa P")baa# ."#"&'%

P")baa# ))&a# DNA (aa& m"#$"bab%a#

")baa# a(a "%*"*' ."# (a# a&a) m"#.%+("+()% +&"'# $a#. &",a &")ba P")baa# "'."#"&'%

E'."#"&'% m"#..amba%a# m+('%a*' %+ma&'#(a# DNA $a#. m"#.)ba "%*"*' ."# #am)#

&'(a% m",'ba&%a# ")baa# a(a ))&a# DNA$a#. b"*a#.%)&a#!K"a(aa# m'&+*'* *&ab', ("#.a# ")baa# a%&'


13/30

TIN@AUAN PUSTAKA

>"#.%" (Syzygium aromaticum (L.)Merr & Perr)

Gamba ! P++#>"#.%"


14/30

>"#.%" (Syzygium aromaticum (L.) Merr& Perr) Ma#aa& "#.+ba&a# b"'-b"' #$"' ")&

,"ma *$a;a& *"ba.a' +ba& a(a#. m"mba#m)%+*a m),)& (a# &"#..++%a# b)#.a#$a('.)#a%a# (a,am '#()*&' +%+% (a# '#()*&'ma%a#a# m'#$a% 8"#.%" ('ma#aa&%a# +,"

'#()*&' ama*' %+*m"&'% (a# *"ba.a'#$a Ka#()#.a# m'#$a% 8"#.%" m'#$a% a&*''3

")."#+, 06-51C3 a*"&', ")."#+, %a'+","#

TIN@AUAN PUSTAKA


15/30


16/30

TIN@AUAN PUSTAKA

M>9-7 D'"+," (a' ")*'

,")a

a("#+%a*'#+maa$)(aa

"*"&+ "*&+."#+*'&'

T47D D'"+," (a' ")*'

,")a

Ka*'#+ma ()%&a, $a#.'#


17/30

Eugenol('&"m)%a# (aa& m"#.'#()%*'a+&+*'* a(a *", ma*& *", m",a#+ma*"&a *", ,")%"m'a! Eugenolm")a%a#

%+m+#"# )&ama (a' m'#$a% 8"#.%"!S"#$a;a &))#a# isofaon 57-(''(+%*'-=-4=-('m"&+%*''*+a9-7 (a# T47D

LANDASAN TEORI


18/30

HIPOTESIS

S"#$a;a &))#a# isofaon 57-

(''(+%*'-=-4=-('m"&+%*''*+a


19/30

KERANGKA TEORI

disintesis dari diduga memiliki

mempunyai

Gambar 5. Kerangka teori aktivitas sitotoksik senyawa turunan isoflavon, 5,7-dihidroksi-

3--dimetoksiiso!lavon yang disintesis dari ekstraksi eugenolminyak daun "engkeh

terhadap cell linekanker payudara, #$%-7 dan &7'


20/30

KERANGKA KONSEP

Gambar (. Kerangka konsep variabel penelitian aktivitas sitotoksik senyawa turunan

isoflavon, 5,7-dihidroksi-3--dimetoksiiso!lavon yang disintesis dari ekstraksi eugenolminyak daun "engkeh terhadap cell linekanker payudara, #$%-7 dan &7'


21/30

S)b"% P"#",'&'a#

P+),a*' K),&) cell line%a#%" a$)(aa M>9-7 (a#T47D

Sam", S)*"#*' *", M>9-7 a&a) T47D (a,am m"('a

%),&) ("#.a# %"a(a&a# 15 104

*",/100L (a,am ma*'#.-ma*'#. *)m)a#

micro!ell plate3 F6 (a# (''#%)ba*' a(a'#%)ba&+ *)) 70 > 5C >O2*",ama 24 am


22/30


23/30

A,a& (a# Baa#

Micropipette,&ab)#. "a%*' counting c"am$er(improed %eu$auer) coer glass m'%+*%+a,a& "#.'&)#. *", '#%)ba&+ micro!ell plate#"a8a &'mba#.a# *"#&').a&+ mier3 ELISA

reader! Baa# )' b")a ,a)&a# *&+% *"#$a;a )' 57-

(''(+%*'-=4=-('m"&+%*''*+a


24/30


25/30

:ARIABEL DATA YANG AKANDITELITI

:a'ab", b"ba* a(a,a %+#*"#&a*',a)&a# *"#$a;a 57-(''(+%*'-=-4=-

('m"&+%*''*+a9-7 (a# T47D

$a#. ('&)#)%%a# ("#.a# #',a' I>50!


26/30

DE9INI OPERASIONAL

K+#*"#&a*' ,a)&a# *"#$a;a 57-(''(+%*'-=-4=-('m"&+%*''*+a50

$a#. ('(aa& (a' "'&)#.a# a#a,'*'*+b'& b"(a*a%a# )b)#.a# a#&aa %a(a("#.a# "#.amba&a# "&)mb)a# *",!


27/30

ANALISIS HASIL

Da' a*', a#a,'*'* ab*+ba#*' ma%a (aa&(''&)#. "*"#&a*" "#.amba&a#"&)mb)a# *", ("#.a# )m)*

P"*"#&a*" "#.amba&a# )m,a "&)mb)a#*", a-b3-8-b3 100C

K"&"a#.a#

a ab*+ba#*' %+#&+, *",b ab*+ba#*' %+#&+, m"('a

8 ab*+ba#*' ma*'#.-ma*'#. %+#*"#&a*'*"#$a;a )'


28/30

A%&'


29/30

@ADJAL KEGIATAN

Wak! K"#$aa%

N+


30/30

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