Page 1
LAMPIRAN
Lampiran 1 Data jumlah AgNOR pada seluruh kelompok
Kontrol 1
3 5 7 5 4 7 6 7 6 6 Jumlah8 5 4 5 5 5 9 5 6 5
611
6 5 4 5 6 4 6 6 5 77 5 6 5 6 8 6 6 5 79 8 10 8 5 9 9 8 7 96 4 5 4 8 4 9 4 5 76 4 7 8 6 5 6 7 7 54 4 6 4 6 7 6 4 8 56 4 5 5 9 7 5 6 6 46 7 11 7 8 11 5 4 7 7
Rata rata 6,11
Kontrol 2
5 5 7 5 7 5 7 6 10 14 Jumlah5 7 8 5 5 5 6 9 9 10
740
11 11 5 10 10 11 7 8 5 37 6 8 8 14 11 8 6 5 66 7 6 5 6 6 7 6 11 87 5 8 7 6 8 10 4 6 66 5 9 5 6 6 5 6 9 96 11 6 5 6 6 6 4 9 9
11 7 8 12 9 9 9 8 11 94 7 8 7 6 5 11 10 8 12
Rata rata 7,4
Kontrol 3
6 10 4 6 8 7 5 6 6 6 Jumlah10 6 4 7 6 6 4 10 10 4
631
10 10 9 6 4 6 4 6 8 86 4 6 7 10 6 4 8 4 44 4 4 8 4 6 4 10 6 6
10 4 4 8 4 6 6 4 8 44 10 6 5 4 6 4 10 6 67 6 6 8 6 6 4 4 10 4
10 6 10 6 6 8 4 5 4 108 6 8 6 8 8 4 6 7 4
Rata rata 6,31
Kontrol 4 4 4 8 4 4 4 4 4 8 9 Jumlah8 4 7 4 4 4 4 4 6 4
518
8 4 8 6 6 6 5 4 8 47 5 7 10 6 4 4 4 4 66 4 8 10 6 6 4 4 5 44 8 6 4 4 5 4 4 6 4
Page 2
8 4 4 6 6 4 10 4 10 44 4 4 4 4 4 4 4 4 44 4 4 4 5 4 4 4 4 5
8 4 4 4 6 4 10 4 4 4Rata rata 5,18
Kontrol 5
4 5 4 4 3 6 6 7 7 5 Jumlah5 5 7 5 4 4 3 4 6 7
568
3 5 5 3 8 6 3 4 8 66 5 4 4 6 5 7 6 4 64 5 4 5 3 6 7 5 6 83 5 4 4 4 6 4 6 7 94 4 7 4 6 4 5 6 7 74 4 5 6 5 4 8 6 5 77 6 8 7 7 9 7 8 6 79 7 6 8 10 7 6 8 7 10
Rata rata 5,68
Kontrol 6
4 4 10 10 8 8 6 4 8 4 Jumlah4 6 6 6 4 4 10 8 6 10
658
4 6 4 6 6 6 8 6 4 66 6 10 10 4 4 8 6 6 10
10 10 8 10 4 8 6 4 8 86 10 10 6 8 6 4 4 8 66 6 10 6 6 6 4 8 4 66 10 6 10 6 8 6 4 6 48 10 6 8 6 6 8 6 4 44 6 10 10 6 4 8 4 8 4
Rata rata 6,58
Page 3
Dosis 1,1
5 7 4 2 4 4 5 2 2 5 Jumlah2 4 3 2 4 5 4 2 2 4
329
3 6 5 3 6 2 2 3 2 43 3 3 3 3 5 4 2 1 32 4 4 3 2 2 2 4 1 45 9 3 4 1 5 2 3 4 44 4 3 4 4 6 2 4 4 32 5 5 3 3 2 2 1 2 3
Page 4
2 4 3 3 2 2 5 3 3 42 3 2 2 3 3 3 3 3 2
Rata rata 3,29
Dosis 1,2
7 4 6 5 5 5 4 4 6 4 Jumlah4 3 5 4 9 5 4 3 6 4
488
4 5 3 8 5 4 4 3 4 63 6 5 7 7 4 9 4 3 65 4 7 5 5 5 4 3 3 64 6 5 5 6 4 6 5 9 44 4 4 3 8 5 4 4 7 56 4 8 5 4 4 6 4 8 86 3 4 4 8 4 5 6 8 54 3 3 3 4 3 3 3 3 3
Rata rata 4,88
Dosis 1,3
3 2 5 2 3 4 4 3 4 5 Jumlah3 5 4 2 3 3 4 3 3 3
346
2 2 4 5 3 4 4 4 3 34 3 4 2 4 4 9 4 3 23 5 3 3 6 2 3 4 4 46 2 5 2 2 3 5 6 4 62 2 2 2 2 3 3 3 2 23 2 4 5 3 3 2 4 4 23 3 5 2 4 3 2 4 4 24 3 3 3 6 3 5 3 4 6
Rata rata 3,46Dosis 1,4 3 8 4 4 6 5 4 5 5 9 Jumlah
4 10 4 3 4 7 6 4 5 4
490
4 7 5 3 6 5 4 4 3 63 6 4 5 7 4 7 8 5 54 4 3 3 4 3 3 7 7 64 8 5 7 5 4 6 4 11 73 3 5 8 5 4 3 6 6 83 6 5 8 4 7 3 5 7 83 3 3 7 4 3 5 8 4 52 2 4 3 3 3 3 2 3 3
Rata rata 4,9
Dosis 1,5
4 3 5 6 7 4 7 5 5 6 Jumlah4 4 6 8 7 4 6 3 9 5
498
3 3 7 6 5 6 6 5 8 64 3 3 5 3 5 4 4 4 54 6 4 3 4 3 7 4 7 75 3 4 5 6 5 3 4 4 9
Page 5
8 3 3 5 8 5 7 4 4 53 5 9 3 5 6 3 4 9 135 8 5 4 8 5 4 3 6 54 4 3 3 4 3 3 3 4 2
Rata rata 4,98
Dosis 1,6
4 3 6 4 4 3 7 3 3 4 Jumlah5 5 5 3 3 3 5 3 3 7
404
3 5 4 4 4 4 3 5 3 25 4 3 6 4 4 4 3 3 64 4 4 3 5 6 6 4 2 32 2 3 3 8 6 5 4 6 54 5 5 5 4 4 4 3 7 52 4 6 4 7 6 3 4 2 62 3 3 5 6 6 4 2 3 73 3 2 3 2 3 3 2 2 3
Rata rata 4,04
Dosis 2,1
5 5 4 5 4 7 2 3 4 2 Jumlah8 6 6 4 3 4 5 4 6 7
398
5 3 6 3 4 7 6 3 4 66 2 3 5 2 3 3 4 4 64 5 3 2 2 1 4 4 3 75 4 5 3 4 6 2 3 2 63 3 4 2 4 3 5 5 6 75 3 3 3 4 6 3 2 4 53 5 5 2 6 5 2 4 2 22 3 2 4 3 3 2 4 2 3
Rata rata 3,98
Dosis 2,2
3 5 7 4 3 2 7 3 5 4 Jumlah7 5 4 3 6 3 6 1 3 4
432
6 7 7 1 5 4 4 3 5 87 9 7 5 3 4 7 5 2 45 4 3 5 3 7 1 1 4 37 5 7 4 5 9 8 3 5 28 3 6 4 3 4 7 6 3 26 5 6 3 3 3 7 2 5 14 8 5 3 5 2 4 1 3 42 3 4 2 2 2 2 3 4 3
Rata rata 4,32
Dosis 2,35 8 4 3 3 3 3 12 6 2 Jumlah3 4 9 3 2 2 6 7 6 8
4465 4 6 3 3 5 5 8 10 5
Page 6
6 4 4 6 9 4 4 3 3 35 6 5 3 3 3 5 4 8 13 6 7 2 2 6 3 3 5 24 4 7 4 4 7 1 4 4 16 10 5 3 3 3 5 6 7 33 9 7 5 3 3 4 6 4 23 3 2 4 3 1 3 4 2 3
Rata rata 4,46
Dosis 2,4
2 1 1 1 4 1 1 1 1 1 Jumlah1 1 1 1 4 1 2 2 1 1
145
1 1 2 2 1 1 1 1 3 11 2 1 1 3 2 1 1 1 11 1 1 1 1 1 1 2 1 51 1 2 3 1 1 1 1 2 31 1 1 1 2 1 1 2 1 11 1 1 1 1 4 1 2 1 11 1 1 1 1 1 1 1 2 42 2 2 1 2 2 2 1 2 1
Rata rata 1,45
Dosis 2,5
3 2 2 1 4 2 3 4 2 3 Jumlah1 3 2 1 2 3 2 2 5 1
273
2 4 3 2 2 2 2 3 5 34 4 3 2 3 2 3 4 5 13 4 3 3 4 1 3 2 4 23 3 4 3 4 2 3 3 2 35 1 2 2 2 1 1 4 1 41 6 4 3 4 2 3 4 3 12 5 4 3 2 5 4 2 4 32 2 3 2 1 2 1 1 3 2
Rata rata 2,73
Dosis 2,6
1 1 2 3 1 1 1 3 1 1 Jumlah1 1 1 2 1 1 3 2 2 1
160
1 1 1 3 1 2 2 1 3 21 1 2 1 1 1 1 3 2 31 3 1 2 2 2 2 2 1 31 1 1 1 1 2 1 2 1 12 1 1 1 3 2 3 3 1 32 3 2 1 4 1 1 1 1 31 4 1 1 2 1 1 1 1 11 1 1 2 2 1 1 1 1 2
Rata rata 1,6Dosis 3,1 6 2 4 4 3 1 3 1 6 4 Jumlah
Page 7
5 5 5 3 3 2 4 4 4 6
308
3 4 4 2 2 1 3 1 1 46 3 5 4 3 2 3 1 4 23 3 6 6 5 2 2 5 2 32 3 2 2 2 2 5 2 1 53 3 2 1 2 2 3 2 3 53 5 4 4 6 2 3 4 5 33 4 2 1 4 2 3 2 4 43 1 3 2 2 1 2 1 1 2
Rata rata 3,08
Dosis 3,2
1 1 2 4 2 5 1 1 1 1 Jumlah4 4 1 5 3 4 4 1 1 2
229
1 4 1 3 2 3 1 3 4 21 3 2 1 1 1 1 3 4 11 2 6 1 2 2 2 2 2 22 2 4 1 3 2 3 1 2 21 2 4 3 3 3 2 1 1 43 2 6 3 2 3 5 1 1 11 1 1 5 2 1 6 3 4 21 3 2 2 3 1 1 2 2 1
Rata rata 2,29
Dosis 3,3
7 3 8 3 3 12 2 2 4 3 Jumlah5 2 8 8 7 6 5 6 2 3 4154 2 7 7 5 4 5 3 2 33 6 6 7 4 6 4 3 2 44 3 8 6 7 6 4 3 3 43 2 5 7 4 4 7 8 1 24 1 5 5 3 6 2 1 4 26 4 2 5 5 7 3 4 2 44 5 9 3 2 6 3 3 4 32 3 1 3 2 2 1 2 2 3
Rata rata 4,15
Dosis 3,4
2 5 2 2 4 3 3 3 4 5 Jumlah2 3 4 4 3 5 1 6 5 6
377
2 2 6 4 2 4 6 5 5 66 6 6 3 2 3 7 4 3 64 9 7 4 3 6 7 3 3 72 5 2 3 4 4 4 2 4 42 4 3 2 4 6 6 2 3 74 1 4 2 2 1 3 4 3 65 6 1 2 3 3 4 7 5 52 2 2 1 3 2 1 2 3 2
Page 8
Rata rata 3,77
Dosis 3,5
3 3 4 2 1 3 4 3 3 5 Jumlah3 3 1 2 2 1 3 2 2 3
279
4 3 3 3 2 2 3 2 2 24 3 3 4 2 5 6 2 2 35 2 3 2 2 1 3 3 1 32 3 2 2 3 3 1 3 4 33 3 3 2 4 5 4 2 3 49 1 2 5 3 3 1 2 2 25 4 6 3 3 3 2 4 3 42 2 2 1 2 1 1 1 2 1
Rata rata 2,79
Dosis 3,6
2 5 2 2 4 3 3 3 4 5 Jumlah2 3 4 4 3 5 1 6 5 6
377
2 2 6 4 2 4 6 5 5 66 7 7 3 2 3 6 4 3 64 7 7 4 3 6 7 3 3 72 5 2 3 4 4 4 2 4 42 4 3 2 4 6 6 2 3 74 1 4 2 2 1 3 4 3 65 7 1 2 3 3 4 7 5 52 2 2 1 3 2 1 2 3 2
Rata rata 3,77
Lampiran 2 Descriptive Test Sebelum transformasi
Descriptives
Dosis Statistic Std. Error
agnor kontrol Mean 6,2100 ,31170
95% Confidence Interval for
Mean
Lower Bound 5,4087
Upper Bound 7,0113
5% Trimmed Mean 6,2011
Median 6,2100
Variance ,583
Std. Deviation ,76352
Minimum 5,18
Maximum 7,40
Range 2,22
Page 9
Interquartile Range 1,23
Skewness ,333 ,845
Kurtosis ,400 1,741
dosis 1
Mean 4,1567 ,28688
95% Confidence Interval for
Mean
Lower Bound 3,4192
Upper Bound 4,8941
5% Trimmed Mean 4,1591
Median 4,1650
Variance ,494
Std. Deviation ,70270
Minimum 3,29
Maximum 4,98
Range 1,69
Interquartile Range 1,49
Skewness -,045 ,845
Kurtosis -1,847 1,741
dosis 2
Mean 3,0900 ,55441
95% Confidence Interval for
Mean
Lower Bound 1,6648
Upper Bound 4,5152
5% Trimmed Mean 3,1050
Median 3,3550
Variance 1,844
Std. Deviation 1,35803
Minimum 1,45
Maximum 4,46
Range 3,01
Interquartile Range 2,79
Skewness -,315 ,845
Kurtosis -2,418 1,741
dosis 3 Mean 3,3083 ,28824
95% Confidence Interval for
Mean
Lower Bound 2,5674
Upper Bound 4,0493
5% Trimmed Mean 3,3181
Median 3,4250
Variance ,498
Page 10
Std. Deviation ,70604
Minimum 2,29
Maximum 4,15
Range 1,86
Interquartile Range 1,20
Skewness -,353 ,845
Kurtosis -1,340 1,741
Lampiran 3 Test of Normality Sebelum transformasi
Tests of Normality
dosis Kolmogorov-Smirnova Shapiro-Wilk
Statistic Df Sig. Statistic df Sig.
agnor
kontrol ,147 6 ,200* ,989 6 ,986
dosis 1 ,182 6 ,200* ,920 6 ,507
dosis 2 ,244 6 ,200* ,856 6 ,175
dosis 3 ,243 6 ,200* ,942 6 ,672
*. This is a lower bound of the true significance.
a. Lilliefors Significance Correction
Page 11
Lampiran 4 Homogenity Test Sebelum transformasi
Test of Homogeneity of Variance
Levene Statistic df1 df2 Sig.
agnor
Based on Mean 3,359 3 20 ,039
Based on Median 2,900 3 20 ,060
Based on Median and with
adjusted df
2,900 3 16,676 ,066
Based on trimmed mean 3,356 3 20 ,039
Page 12
Lampiran 5 Test of Normality Setelah transformasi
Tests of Normality
dosis Kolmogorov-Smirnova Shapiro-Wilk
Statistic Df Sig. Statistic Df Sig.
trans_agnor
kontrol ,163 6 ,200* ,976 6 ,930
dosis 1 ,186 6 ,200* ,917 6 ,482
dosis 2 ,223 6 ,200* ,860 6 ,190
dosis 3 ,234 6 ,200* ,944 6 ,689
*. This is a lower bound of the true significance.
a. Lilliefors Significance Correction
Page 13
Lampiran 6 Homogenity Test Setelah transformasi
Test of Homogeneity of Variance
Levene Statistic df1 df2 Sig.
trans_agnor
Based on Mean 1,195 3 20 ,337
Based on Median 1,171 3 20 ,346
Based on Median and with
adjusted df
1,171 3 10,086 ,369
Based on trimmed mean 1,194 3 20 ,338
Page 14
Lampiran 7 One Way Anova Test
ANOVA
trans_agnor
Sum of Squares Df Mean Square F Sig.
Between Groups 3133,144 3 1044,381 19,659 ,000
Within Groups 1062,493 20 53,125
Total 4195,637 23
Page 15
Lampiran 8 Post Hoc Bonferroni
Multiple Comparisons
Dependent Variable: trans_agnor
Bonferroni
(I) dosis (J) dosis Mean Difference
(I-J)
Std. Error Sig. 95% Confidence Interval
Lower Bound Upper Bound
kontrol
dosis 1 21,36053* 4,20811 ,000 9,0429 33,6782
dosis 2 27,96493* 4,20811 ,000 15,6473 40,2826
dosis 3 ,68942* 4,20811 ,000 15,3718 40,0071
dosis 1
kontrol -21,36053* 4,20811 ,000 -33,6782 -9,0429
dosis 2 6,60440 4,20811 ,793 -5,7133 18,9221
dosis 3 6,32888 4,20811 ,889 -5,9888 18,6465
dosis 2
kontrol -27,96493* 4,20811 ,000 -40,2826 -15,6473
dosis 1 -6,60440 4,20811 ,793 -18,9221 5,7133
dosis 3 -,27552 4,20811 1,000 -12,5932 12,0421
Page 16
dosis 3
kotrol -27,68942* 4,20811 ,000 -40,0071 -15,3718
dosis 1 -6,32888 4,20811 ,889 -18,6465 5,9888
dosis 2 ,27552 4,20811 1,000 -12,0421 12,5932
*. The mean difference is significant at the 0.05 level.
Lampiran 9 Dokumentasi Penelitian
Gambar 1. Mencit donor
Page 17
Gambar 2. Anastesi mencit donor
Gambar 3. Persiapan pembedahan mencit donor
Page 18
Gambar 4. Pembedahan mencit donor
Gambar 5. Tumor
Page 19
Gambar 6. Pembuatan bubur tumor
Gambar 7. Transplantasi bubur tumor ke mencit resipien
Page 20
Gambar 8. Pemberian ekstrak Sarang Semut dan Methotrexate melalui sonde
Gambar 9. Preparat kelompok Kontrol, perbesaran 1000x dengan pengecatan AgNOR
Bintik AgNOR
Bintik AgNOR
Page 21
Gambar 10. Preparat kelompok dosis 1, perbesaran 1000x dengan PengecetanAgNOR
Gambar 11. Preparat kelompok dosis 2, perbesaran 1000x dengan pengecatan AgNOR
Bintik AgNOR
Page 22
Gambar 12. Preparat kelompok dosis 3, perbesaran 1000x dengan pengecatan AgNOR
Lampiran 10. Surat Ijin Penelitian
Bintik AgNOR
Page 23
Lampiran 11. Surat Keterangan Penelitian dari Departemen Patologi Anatomik FK UI
Page 24
Lampiran 12. Surat Ijin Penelitian Laboratorium Patologi Anatomi FK
UNISSULA
Page 25
Lampiran 13.Ethical Clearance