[06] Klasifikasi Morfologi Replikasi Virus (Nurul Wiqoyah, Dra.,MSi) 27-02-2013

Nurul Wiqoyah

Klasifikasi dan identifikasi virus

Awal 1900an, virus dikelompokkan tersendiri Identifikasi:Karakteristik biologi, morfologi, struktur genom, sifat antigenik

Morfologi: ukuran, bentuk, capsid symmetry, ada tidaknya envelop

Sifat fisik virion,

struktur genom

lipid pattern, KH, protein struktural dan non struktural,\

sifat antigenik dan biologi , strategi replikasi , host range, mode of transmission dan patogenitas

Klasifikasi

Ordo ---- viralesFamili ---- viridaeSubfamili ---- virinae Genera ---- virusSpecies

Taxonomy Ordo MononegairalesOrder Family Subfamily Genus Type Species Hosta Mononegavirales Bornaviridae Bornavirus Borna disease virus V Rhabdoviridae Vesiculovirus Vesicular stomatitis Indiana virus V, I Lyssavirus Rabies virus V Ephemerovirus Bovine ephemeral fever virus V, I Novirhabdovirus Infectious hematopoietic necrosis virus V Cytorhabdovirus Lettuce necrotic yellows virus P, I Nucleorhabdovirus Potato yellow dwarf virus P, I Filoviridae Marburghvirus Lake Victoria marburgvirus V Ebolavirus Zaire ebolavirus V Paramyxoviridae Paramyxovirinae Rubulavirus Mumps virus V Avulavirus Newcastle disease virus V Respirovirus Sendai virus V Henipavirus Hendra virus V Morbillivirus Measles virus V Pneumovirinae Pneumovirus Human respiratory syncytial virus V Metapneumovirus Avian metapneumovirus V

V, vertebrate; I, insect; P, plant.

Fields Vriology (2007) 5th edition, Knipe, DM & Howley, PM, eds, Wolters Kluwer/Lippincott Williams & Wilkins, Philadelphia Table 2.2

Klasifikasi virus manusia"Group" Family Genome Genome size (kb) Capsid EnvelopedsDNA

Poxviridae dsDNA, linear 130 to 375 Ovoid YesHerpesviridae dsDNA, linear 125 to 240 Icosahedral YesAdenoviridae dsDNA, linear 26 to 45 Icosahedral NoPolyomaviridae dsDNA, circular 5 Icosahedral NoPapillomaviridae dsDNA, circular 7 to 8 Icosahedral No

ssDNAAnellovirus ssDNA circular 3 to 4 Isometric NoParvoviradae ssDNA, linear, (- or +/-) 5 Icosahedral No

RetroHepadnaviridae dsDNA (partial), circular 3 to 4 Icosahedral YesRetroviridae ssRNA (+), diploid 7 to 13 Spherical, rod or cone shaped Yes

dsRNAReoviridae dsRNA, segmented 19 to 32 Icosahedral No

ssRNA (-)Rhabdoviridae ssRNA (-) 11 to 15 Helical YesFiloviridae ssRNA (-) 19 Helical YesParamyxoviridae ssRNA (-) 10 to 15 Helical YesOrthomyxoviridae ssRNA (-), segmented 10 to 13.6 Helical YesBunyaviridae ssRNA (-, ambi), segmented 11 to 19 Helical YesArenaviridae ssRNA (-, ambi), segmented 11 Circular, nucleosomal YesDeltavirus ssRNA (-) circular 2 Spherical Yes

ssRNA (+)Picornaviridae ssRNA (+) 7 to 9 Icosahedral NoCalciviridae ssRNA (+) 7 to 8 Icosahedral NoHepevirus ssRNA (+) 7 Icosahedral NoAstroviridae ssRNA (+) 6 to 7 Isometric NoCoronaviridae ssRNA (+) 28 to 31 Helical YesFlaviviridae ssRNA (+) 10 to 12 Spherical YesTogaviridae ssRNA (+) 11 to 12 Icosahedral Yes

Fields Vriology (2007) 5th edition, Knipe, DM & Howley, PM, eds, Wolters Kluwer/Lippincott Williams & Wilkins, Philadelphia Table 2.1

Struktur dasar virus

DNA/ RNA + Capsid protein ---Nucleocapsid ---Naked virus

Nucleocapsid + lipid membran, glicoprotein --- enveloped virus

Brock Biology of Microorganism, 2012, M.T. Madigan et al

Icosahedral Cymmetry

Icosahedral

A, Human papilloma Virus (HPV), B. Tiga dimensi HPV

Helix

Helical Naked Capsid Virus

Bacteriophage

Virology, principle and Application, 2007, John Carter, V, Saunders

Replikasi virus4 fase dasar:

AttachmentEntry/ PenetrationGenome uncoatingGenome replication and protein synthesisAssembling progenyExit/ release

Attachment dan entry

Virology, principle and Application, 2007, John Carter, V, Saunders

Reseptor sel, protein virus dalam attachment

Virus Reseptor sel Protein yang terlibat dalam

Attachment ke reseptor

Fusi

Naked virus90% Rhinovirus mns

Poliovirus

Intercellular mol adhesion 1 (ICAM 1)CD155

VP1 +VP3

VP1

HIV-1 CD4 Gp120 Gp41

Virus influenzae A,B

Glycoprotein yang mengandung sialic acid

Haemagglutinin Haemagglutinin

Genome uncoating

Tergantung Virus, proses dapat terjadi:

1. pada permukaan sel, capsid tetap di permukaan sel 2. dalam sitoplasma3. dalam pori nukleus4. dalam nukleus

Assembly dan release

Virology, principle and Application, 2007, John Carter, V, Saunders